Protective effects of Passiflora Incarnata on ischemia-reperfusion injury in testicular torsion: an experimental study in a rat model




Mustafa Azizoğlu, Clinic of Pediatric Surgery, Esenyurt Necmi Kadıoğlu State Hospital, Istanbul, Turkey
Serkan Arslan, Department of Pediatric Surgery, Dicle University Medical School, Diyarbakır, Turkey
Ebru Gökalp-Özkorkmaz, Department of Nursing, Faculty of Health Sciences, Yıldırım Beyazit University, Ankara, Turkey
Fırat Aşır, Department of Histology and Embryology, Medical School, Dicle University, Diyarbakır, Turkey
Erol Basuguy, Department of Pediatric Surgery, Dicle University Medical School, Diyarbakır, Turkey
Mehmet H. Okur, Department of Pediatric Surgery, Dicle University Medical School, Diyarbakır, Turkey
Bahattin Aydoğdu, Department of pediatric surgery, Balıkesir University Medical School, Balıkesir. Turkey
Müsemma A. Karabel, Department of Pediatrics and Pediatric Pneumology, Dicle University Medical School, Diyarbakır, Turkey
Mansur Dağgülli, Department of Urology, Medical School, Dicle University, Diyarbakır, Turkey
İbrahim Kaplan, Department of Biochemistry, Medical School, Dicle University, Diyarbakır. Turkey


Objective: The current study aimed to explore the potential protective effect of Passiflora Incarnata L., (PI) in treating IR injury after testicular torsion in rats. Materials and methods: This research investigated the impact of PI on IR damage in male Wistar albino rats. Animals were divided to three groups: group 1 (sham), group 2 (IR), and group 3 (IR+PI). Results: The malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione (GSH) levels did not significantly differ across the groups (p = 0.830, p = 0.153 and p=0.140, respectively). However, Group 3 demonstrated a superior total antioxidant status (TAS) value compared to Group 2 (p = 0.020). Concurrently, Group 3 presented a significantly diminished mean total oxidant status (TOS) relative to Group 2 (p = 0.009). Furthermore, Group 3 showed a markedly improved Johnsen score relative to Group 2 (p < 0.01). IR caused cell degeneration, apoptosis, and fibrosis in testicular tissues. PI treatment, however, mitigated these effects, preserved seminiferous tubule integrity and promoted regular spermatogenesis. Furthermore, it reduced expression of tumor necrosis factorâ??alpha (TNF-α), Bax, and Annexin V, signifying diminished inflammation and apoptosis, thereby supporting cell survival (p < 0.01, p < 0.01, p < 0.01, respectively). Conclusions: This study revealed that PI significantly reduces oxidative stress and testicular damage, potentially benefiting therapies for IR injuries.



Keywords: Passiflora Incarnata. Ischemia reperfusion. Testicular torsion. Testicular damage. Spermatogenesis.