Melih Canli, Department of Plastic, Reconstructive and Aesthetic Surgery, Gulhane Faculty of Medicine, University of Health Sciences, Ankara, Turkey
Nesrin Tan Baser, Department of Plastic, Reconstructive and Aesthetic Surgery, Gulhane Faculty of Medicine, University of Health Sciences, Ankara, Turkey
Simay Ersahin, Department of Plastic, Reconstructive and Aesthetic Surgery, Bitlis Tatvan State Hospital, Bitlis, Turkey
Ceren Bilgin-Bora, Department of Medical Pathology, Gulhane Faculty of Medicine, University of Health Sciences, Ankara, Turkey
Gulcin Simsek, Department of Medical Pathology, Gulhane Faculty of Medicine, University of Health Sciences, Ankara, Turkey
Objective: The present study aimed to investigate the effects of oleanolic acid (OA) on I/R injury in a rat groin flap model. Method: 21 male Wistar albino rats were randomly divided into three groups (n = 7): Group I (sham), Group II (I/R), and Group III (OA+I/R). OA was administered intraperitoneally for five pre-operative days. Flap elevation was performed on the 6th day, and the subjects were exposed to 2 h of ischemia followed by reperfusion. Biochemical and histopathological parameters were evaluated. Results: Group 3 presented a significantly diminished mean total oxidant stress relative to Group 2 (p = 0.002). Furthermore, a significant increase in total antioxidant capacity levels was observed in Group 3 compared to Group 2 (p = 0.048). There was no statistically significant difference between the groups in terms of inflammation and edema (p > 0.05). Necrosis did not develop in any of the samples. Conclusion: This study revealed that OA significantly reduces oxidative stress and increases antioxidant capacity, potentially benefiting therapies for I/R injuries.
Keywords: Oleanolic acid. Ischemia. Reperfusion injury.