Ünal T. Öztürk, Department of Pediatric Surgery, Artvin Public Hospital, Artvin. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Hatice S.Y. Cömert, Department of Pediatric Surgery. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Gül Şalcı, Department of Pediatric Surgery. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Ahmet Alver, Department of Biochemistry. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Sevdegül A. Mungan, Department of Pathology. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Neslihan Sağlam, Department of Biochemistry. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Şeniz Erdem, Department of Biochemistry. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Serdar Karakullukçu, Department of Public Health. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Mustafa İmamoğlu, Department of Pediatric Surgery. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Haluk Sarıhan, Department of Pediatric Surgery. Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
Objective: Our aim was to examine the effectiveness of human breast milk exosomes in treating testicular torsion.
Methods: Rats were divided randomly into three equal groups. Group 1 was our control group. A torsion/detorsion model was created for the rats in Group 2. After the torsion/detorsion model, 1 mg/kg intraperitoneally human breast milk exosomes were given 30 min before detorsion in Group 3. All testicles were removed at the 6th h of the experiment, for biochemical and histopathological examination.
Results: There was a statistically significant decrease in Group 3 MDA values compared to Group 2. When Group 3 and Group 2 SOD and CAT values were compared, it was seen that these values were higher in Group 3. Histopathologically a statistically significant difference was found in Group 3 compared to Group 1 and Group 2 in terms of ischemia/reperfusion injury.
Conclusion: We have shown that human breast milk exosomes in testicular torsion do not completely prevent reperfusion injury but significantly preserve testicular tissue.
Keywords: Testicular torsion; Exosome; Ischemia/reperfusion